Rapid labeling of lipoproteins in plasma with radioactive cholesterol. Application for measurement of plasma cholesterol esterification.
نویسندگان
چکیده
منابع مشابه
Cholesterol esterification in rabbit plasma.
1. When [4-(14)C]cholesterol, attached to beta-globulin or dispersed with Tween 20, was incubated with fresh rabbit (New Zealand albino females) plasma, 30-47% esterification was observed. The optimum pH was 6.8. This esterification was accomplished by the transfer of fatty acids from the C-2 position of lecithin (phosphatidylcholine) to cholesterol. 2. There was no evidence that triglycerides ...
متن کاملEffect of labeling of plasma lipoproteins with [(3)H]cholesterol on values of esterification rate of cholesterol in apolipoprotein B-depleted plasma.
The fractional esterification rate of cholesterol in apolipoprotein B (apoB)-depleted plasma (FER(HDL)) is a good indicator of particle size distribution in high density lipoprotein (HDL) and low density lipoprotein (LDL). However, there has been a discrepancy in the absolute values of FER(HDL) published by different laboratories. Because the main difference between the methods was in the label...
متن کاملInitial rate of cholesterol esterification associated with high density lipoproteins in human plasma
The enzyme 1ecithin:cholesterol acyl transferase has been measured both in total plasma and in the fraction of plasma from which very low and low density lipoproteins have been removed by ultracentrifugation. The correlation between the activity of the enzyme and the free cholesterol concentration was positive in whole plasma and negative in apoB-deficient plasma. On the other hand, the positiv...
متن کاملInitial rate of cholesterol esterification association with high density lipoproteins in human plasma.
The enzyme lecithin:cholesterol acyl transferase has been measured both in total plasma and in the fraction of plasma from which very low and low density lipoproteins have been removed by ultracentrifugation. The correlation between the activity of the enzyme and the free cholesterol concentration was positive in whole plasma and negative in apoB-deficient plasma. On the other hand, the positiv...
متن کاملRole of sphingomyelin in the regulation of cholesterol esterification in the plasma lipoproteins. Inhibition of lecithin-cholesterol acyltransferase reaction.
In order to determine whether sphingomyelin (SPH) affects the rate of cholesterol esterification by plasma lecithin-cholesterol acyltransferase (LCAT), we studied the effects of its incorporation in to defined proteoliposome substrates containing phosphatidyl choline (PC), unesterified cholesterol, and apoprotein A-I, on the activity of purified LCAT. Cholesterol esterification was inhibited by...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1990
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)43221-3